| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Caldwell, Hannah M (12)
|
NIVICT |
2:30.75 |
2:31.13 |
0.38 |
| 2 |
Costa, Maria J (12)
|
AMPWRS |
|
2:36.03 |
- |
| 3 |
Barros, Rachel (12)
|
NIUN-B |
2:34.53 |
2:36.59 |
2.06 |
| 4 |
Bojarski, Delaney E (12)
|
AMPWRS |
2:48.15 |
2:40.51 |
-7.64 |
| 5 |
Prezyna, Zoey (11)
|
NIVICT |
2:42.24 |
2:40.85 |
-1.39 |
| 6 |
Borrero, Chloe M (11)
|
AMUN-P |
|
2:42.81 |
- |
| 7 |
Onze, Lauren W (12)
|
NIVICT |
2:49.19 |
2:47.30 |
-1.89 |
| 8 |
Roeser, Lila J (12)
|
NITTSC |
3:15.22 |
2:56.53 |
-18.69 |
| 9 |
Roberts, Haley G (12)
|
NIRAYS |
|
2:57.44 |
- |
| 10 |
Scibetta, Madeline C (12)
|
NITTSC |
3:14.42 |
2:57.97 |
-16.45 |
| 11 |
Huntress, Maya V (11)
|
NIBAAC |
|
2:58.01 |
- |
| 12 |
Strittmatter, Amelia C (11)
|
NISTAR |
3:05.39 |
2:58.85 |
-6.54 |
| 13 |
Weisenburg, Brylee M (12)
|
NISTAR |
3:10.30 |
2:59.71 |
-10.59 |
| 14 |
Lu, Ella (11)
|
NIVELO |
|
3:04.48 |
- |
| 15 |
Tothero, Madeleine S (11)
|
NISTAR |
3:19.01 |
3:05.87 |
-13.14 |
| 16 |
Reukauf, Anna R (11)
|
NISTAR |
3:02.87 |
3:09.32 |
6.45 |
| 17 |
Umansky, Isabella C (11)
|
NIBAAC |
3:10.38 |
3:12.78 |
2.40 |
| 18 |
Zhai, Maggie J (12)
|
NIVELO |
|
3:14.48 |
- |
| 19 |
Urbanek, Addison E (11)
|
NICLSC |
3:14.25 |
3:19.93 |
5.68 |
| 20 |
Haines, Kaylee N (11)
|
NISTAR |
3:32.86 |
3:26.21 |
-6.65 |
| 21 |
Kauffman, Faith M (12)
|
NISTAR |
3:27.12 |
3:27.06 |
-0.06 |
| 22 |
Sidor, Arianna M (11)
|
NITTSC |
|
3:30.94 |
- |
| 23 |
Moch, Hope M (11)
|
NITTAC |
3:47.79 |
3:32.36 |
-15.43 |
|
Jones, Evelyn V (12)
|
NIVELO |
3:47.72 |
DQ |
- |
|
Whitley-Grassi, Ava E (11)
|
NISTAR |
3:11.67 |
DQ |
- |
|
Crawford, Celia A (12)
|
NITTSC |
3:18.32 |
NS |
- |
|
Arcadi, Kayla A (11)
|
NIVELO |
3:18.84 |
NS |
- |