| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Killion, Natalie A (12)
|
NICLSC |
2:23.27 |
2:21.04 |
-2.23 |
| 2 |
Tesluk, Elizabeth D (11)
|
NISTAR |
2:33.61 |
2:31.94 |
-1.67 |
| 3 |
Pathammavong, Suriyathai A (12)
|
NIVICT |
2:33.72 |
2:32.16 |
-1.56 |
| 4 |
Griffin, Zoey O (11)
|
NICLSC |
2:50.26 |
2:35.49 |
-14.77 |
| 5 |
Bidwell, Kylee J (11)
|
NIUN-N |
2:48.47 |
2:41.05 |
-7.42 |
| 6 |
Adamczyk, Gabrielle J (11)
|
NICLSC |
3:02.26 |
2:42.02 |
-20.24 |
| 7 |
Hall, Sofia F (12)
|
NIBAAC |
2:44.37 |
2:42.73 |
-1.64 |
| 8 |
Fragale, Madison J (12)
|
NICLSC |
2:43.69 |
2:43.33 |
-0.36 |
| 9 |
Vaccaro, Vanessa A (12)
|
NIBAAC |
|
2:54.55 |
- |
| 10 |
Gauld, Alison M (12)
|
NISHAQ |
3:34.37 |
3:00.05 |
-34.32 |
| 11 |
Wands, Amelia G (11)
|
NIVELO |
|
3:02.40 |
- |
| 12 |
Mitchell, Gwenyth E (11)
|
NIEMAC |
3:04.21 |
3:03.40 |
-0.81 |
| 13 |
Maloney, Anna R (12)
|
NISTAR |
3:15.10 |
3:12.25 |
-2.85 |
| 14 |
Klose, Shayna (12)
|
NIBAAC |
|
3:23.23 |
- |
| 15 |
Ormond, Erin E (11)
|
NIBAAC |
3:34.68 |
3:40.74 |
6.06 |
| 16 |
Humphries, Hayden S (11)
|
NICLSC |
|
3:43.65 |
- |
|
Lin, Isabella E (11)
|
NIVELO |
2:51.38 |
DQ |
- |
|
Wagner, Mia S (12)
|
NIBAAC |
3:52.41 |
NS |
- |