| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Martino, Adrianna L (12)
|
NIWWSC |
35.99 |
35.66 |
-0.33 |
| 2 |
Bauer, Lauren A (12)
|
NICLSC |
37.34 |
36.18 |
-1.16 |
| 3 |
Kwitek, Grace M (12)
|
NIEAST |
40.46 |
38.03 |
-2.43 |
| 4 |
Hall, Makaila (12)
|
NIVELO |
|
38.55 |
- |
| 5 |
Shedd, Teagan E (12)
|
NISTAR |
36.36 |
38.56 |
2.20 |
| 6 |
Moore, Paige E (12)
|
NIUN-D |
41.82 |
39.48 |
-2.34 |
| 7 |
Rude, Megan R (12)
|
NISHAQ |
55.16 |
41.65 |
-13.51 |
| 8 |
Cronk, Sophia E (12)
|
NICLSC |
|
41.73 |
- |
| 9 |
Zolet, Marta E (12)
|
NIEAST |
47.56 |
41.83 |
-5.73 |
| 10 |
Kowell-Ure, Phoenix W (12)
|
NIEAST |
44.00 |
42.32 |
-1.68 |
| 11 |
McMullen, Haley (12)
|
NIVELO |
44.12 |
43.10 |
-1.02 |
| 12 |
Rietz, Tarin (12)
|
NIVELO |
44.00 |
44.37 |
0.37 |
| 13 |
Asher, Sophia (12)
|
NIVELO |
52.41 |
47.39 |
-5.02 |
| 14 |
Canestaro, Annabelle S (12)
|
NIVELO |
51.66 |
48.23 |
-3.43 |
| 15 |
Hotchkiss, Olivia S (12)
|
NISTAR |
52.27 |
50.80 |
-1.47 |
|
Krough, Mia S (12)
|
NICLSC |
50.09 |
NS |
- |
|
Fix, Grayce R (12)
|
NIWWSC |
51.20 |
NS |
- |