| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Martino, Adrianna L (12)
|
NIWWSC |
38.34 |
35.99 |
-2.35 |
| 2 |
Blake, Emily C (12)
|
NISTAR |
35.54 |
36.11 |
0.57 |
| 3 |
Shedd, Teagan E (12)
|
NISTAR |
38.32 |
36.36 |
-1.96 |
| 4 |
Tesluk, Helen E (12)
|
NISTAR |
39.54 |
40.32 |
0.78 |
| 5 |
Kwitek, Grace M (12)
|
NIEAST |
41.83 |
40.46 |
-1.37 |
| 6 |
LI, Gloria (12)
|
NIUN |
55.00 |
42.47 |
-12.53 |
| 7 |
Vartanova, Sofia (12)
|
NIUN |
55.00 |
45.45 |
-9.55 |
| 8 |
Truong, Jamie L (12)
|
NIUN |
55.00 |
46.66 |
-8.34 |
| 9 |
Hall, Sofia F (12)
|
NIBAAC |
49.25 |
48.77 |
-0.48 |
| 10 |
Naim, Rita (12)
|
NIUN |
59.81 |
49.87 |
-9.94 |
| 11 |
Avila-Shah, Jessi E (12)
|
NIWWSC |
|
49.88 |
- |
| 12 |
Hotchkiss, Olivia S (12)
|
NISTAR |
56.07 |
52.27 |
-3.80 |
| 13 |
Asher, Sophia (12)
|
NIVELO |
|
52.61 |
- |
|
Canestaro, Annabelle S (12)
|
NIVELO |
|
DQ |
- |
|
Zolet, Marta E (12)
|
NIEAST |
47.56 |
NS |
- |