| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fan, Justin F (12)
|
NICLSC |
42.01 |
41.95 |
-0.06 |
| 2 |
LaJeunesse, Peter F (12)
|
NIVELO |
41.57 |
42.32 |
0.75 |
| 3 |
Remick, Spencer S (12)
|
NIBAAC |
44.59 |
43.56 |
-1.03 |
| 4 |
Littrell, Alexander W (12)
|
NISTAR |
43.18 |
44.17 |
0.99 |
| 5 |
Lemmo, Nicholas D (12)
|
NIVELO |
44.65 |
44.48 |
-0.17 |
| 6 |
Coen, Liam C (12)
|
NISTAR |
45.53 |
47.39 |
1.86 |
| 7 |
Meyer, Noah (12)
|
NIVELO |
49.47 |
49.00 |
-0.47 |
| 8 |
Slaughter, Timothy A (12)
|
NIVELO |
54.00 |
51.26 |
-2.74 |
| 9 |
Woodford, Derek (12)
|
NISHAQ |
54.05 |
52.63 |
-1.42 |
| 10 |
Crowther, Eli T (12)
|
NIVELO |
54.29 |
53.82 |
-0.47 |
|
Swan, Ethan M (12)
|
NISTAR |
|
DQ |
- |