| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Deuel, Megan G (14)
|
NIVICT |
2:24.50 |
2:25.37 |
0.87 |
| 2 |
Butler, Victoria A (21)
|
NISTAR |
2:23.16 |
2:28.17 |
5.01 |
| 3 |
Sapio, Olivia M (18)
|
NITWST |
2:22.77 |
2:28.67 |
5.90 |
| 4 |
Webster, Laryssa G (13)
|
NIUN-N |
2:41.22 |
2:34.46 |
-6.76 |
| 5 |
Hawkins, Emma (13)
|
NIVICT |
2:34.04 |
2:34.57 |
0.53 |
| 6 |
Bashor, Allie E (14)
|
NIBAAC |
2:36.46 |
2:36.25 |
-0.21 |
| 7 |
Thomas, Sydney F (18)
|
NISTAR |
2:28.34 |
2:36.40 |
8.06 |
| 8 |
Harris, Gabrielle L (18)
|
NIUN-D |
2:29.77 |
2:37.23 |
7.46 |
| 9 |
Conley, Sarah L (15)
|
NITWST |
2:40.37 |
2:41.37 |
1.00 |
| 10 |
Senglaub, Katie (13)
|
NIVICT |
2:41.78 |
2:45.29 |
3.51 |
| 11 |
McDonnell, Lillian (16)
|
NISTAR |
2:47.16 |
2:47.09 |
-0.07 |
| 12 |
Moore, Madison M (15)
|
NIVICT |
2:38.30 |
2:48.07 |
9.77 |
| 13 |
Studebaker, Gabrielle W (16)
|
NISTAR |
2:54.29 |
2:48.32 |
-5.97 |
| 14 |
Pathammavong, Suriyathai A (12)
|
NIVICT |
3:01.69 |
2:53.05 |
-8.64 |
| 15 |
Riccio, Emma L (15)
|
NISTAR |
2:57.63 |
2:53.10 |
-4.53 |
| 16 |
Drummond, Riley (17)
|
NITWST |
|
2:53.78 |
- |
| 17 |
Van Remmen, Rita A (21)
|
NISTAR |
3:12.00 |
2:57.77 |
-14.23 |
| 18 |
Sapio, Isabel J (16)
|
NITWST |
2:51.47 |
2:58.77 |
7.30 |
| 19 |
Patrier, Tyffany H (17)
|
NIUN-D |
|
3:03.76 |
- |
| 20 |
Tesluk, Helen E (13)
|
NISTAR |
3:08.53 |
3:03.78 |
-4.75 |
| 21 |
Moore, Kathleen E (12)
|
NIUN-D |
3:01.64 |
3:03.94 |
2.30 |
| 22 |
Pasquarella, Paige E (17)
|
NITWST |
3:03.40 |
3:04.24 |
0.84 |
| 23 |
Brodnicki, Brielle T (14)
|
NIVELO |
3:22.53 |
3:21.82 |
-0.71 |