| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wilkie, Kaitlin G (10)
|
NIRAYS |
40.53 |
36.77 |
-3.76 |
| 2 |
Krumpek, Ella E (10)
|
NITWST |
38.58 |
37.00 |
-1.58 |
| 3 |
Rados, Lucy M (9)
|
NITWST |
41.81 |
40.24 |
-1.57 |
| 4 |
Reilly, Bridget F (8)
|
NISTAR |
40.72 |
41.63 |
0.91 |
| 5 |
Zagrobelny, Erin R (9)
|
NIEAST |
45.68 |
42.15 |
-3.53 |
| 6 |
Walters, Elizabeth J (10)
|
NITWST |
43.62 |
42.18 |
-1.44 |
| 7 |
Strittmatter, Amelia C (9)
|
NISTAR |
42.96 |
42.54 |
-0.42 |
| 7 |
Jokic, Sydney G (8)
|
NIWWSC |
45.63 |
42.54 |
-3.09 |
| 9 |
Shunk, Morgan S (9)
|
NICLSC |
44.39 |
43.54 |
-0.85 |
| 10 |
Ziolkowski, Cosette M (10)
|
NITTAC |
43.77 |
44.24 |
0.47 |
| 11 |
Waldon, Hayden E (10)
|
NIGVST |
42.12 |
45.77 |
3.65 |
| 12 |
King, Skylar L (10)
|
NIEAST |
39.85 |
48.81 |
8.96 |
| 13 |
Duff, Rhiannon R (10)
|
NIHAMB |
54.42 |
52.88 |
-1.54 |
| 14 |
Johnson, Mara C (9)
|
NITWST |
58.08 |
57.63 |
-0.45 |