| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wilson, Keara L (9)
|
NEINF |
42.34 |
42.85 |
0.51 |
| 2 |
Demirdag, Zeynep (9)
|
NESTRM |
49.46 |
43.29 |
-6.17 |
| 3 |
Gao, Evie (7)
|
NEMWYS |
44.97 |
43.45 |
-1.52 |
| 4 |
Ebol, Addison A (10)
|
NEINF |
48.90 |
44.41 |
-4.49 |
| 5 |
Dyer, Hayden E (10)
|
NEINF |
46.86 |
45.61 |
-1.25 |
| 6 |
Ho, Caroline R (9)
|
NEMWYS |
45.11 |
46.81 |
1.70 |
| 7 |
Doan, Davina P (10)
|
NESTRM |
52.73 |
47.57 |
-5.16 |
| 8 |
Carline, Brooke A (10)
|
NEHAY |
50.08 |
48.39 |
-1.69 |
| 9 |
Lin, Ayla C (10)
|
NEMWYS |
52.26 |
48.88 |
-3.38 |
| 10 |
Abrahamyan, Sona T (10)
|
NEMWYS |
|
49.10 |
- |
| 11 |
Rogers, Katelyn C (10)
|
NEHAY |
51.59 |
49.51 |
-2.08 |
| 12 |
St. Hilaire, Madison D (10)
|
NEATST |
50.15 |
49.75 |
-0.40 |
| 13 |
Van Corbach, Ashley K (9)
|
NESTRM |
51.40 |
51.25 |
-0.15 |
| 14 |
Bertumen, Darrah E (10)
|
NEHAY |
|
51.41 |
- |
| 15 |
Zhao, Melody S (9)
|
NEATST |
53.89 |
51.64 |
-2.25 |
| 16 |
Savino, Ellie (9)
|
NEINF |
|
52.04 |
- |
| 17 |
Lee, Aila A (10)
|
NEMWYS |
55.31 |
52.12 |
-3.19 |
| 18 |
Kreick, Marleigh R (9)
|
NEINF |
|
52.96 |
- |
| 19 |
Loo, Serena S (8)
|
NEMWYS |
1:08.25 |
53.29 |
-14.96 |
| 20 |
Shea, Madison R (9)
|
NEHAY |
57.61 |
53.59 |
-4.02 |
| 21 |
Sham, Alison M (9)
|
NEMWYS |
53.97 |
54.19 |
0.22 |
| 22 |
Bhushan, Shriya (8)
|
NEHAY |
|
54.31 |
- |
| 23 |
Dufoe, Olivia C (9)
|
NEINF |
55.36 |
56.58 |
1.22 |
| 24 |
DeHoyos, Avery M (7)
|
NESTRM |
1:06.74 |
58.29 |
-8.45 |
| 25 |
Provost, Samantha D (8)
|
NEHAY |
|
58.77 |
- |
| 26 |
Yang, Norah C (7)
|
NEMWYS |
1:10.59 |
1:00.78 |
-9.81 |
| 27 |
Howard, Jillian R (8)
|
NEINF |
|
1:06.60 |
- |
| 28 |
Clark, Kathryn K (9)
|
NEPVAA |
1:14.67 |
1:07.05 |
-7.62 |
| 29 |
Ma, Sakeena H (9)
|
NEMWYS |
|
1:08.77 |
- |
| 30 |
Howard, Evelyn W (7)
|
NEINF |
|
1:11.48 |
- |