| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Brady, Julia M (10)
|
MAGRA |
55.24 |
51.13 |
-4.11 |
| 2 |
Lofgren, Addison E (10)
|
MAACAC |
52.17 |
51.54 |
-0.63 |
| 3 |
Byerley, Adalyn (10)
|
MACAA |
59.11 |
54.52 |
-4.59 |
| 4 |
Coakley, Sloane V (10)
|
MANLAC |
56.60 |
55.66 |
-0.94 |
| 5 |
Kulon, Matylda N (10)
|
MADST |
1:02.21 |
57.25 |
-4.96 |
| 6 |
Steinkirchner, Kaitlin P (10)
|
MACYA |
56.69 |
58.03 |
1.34 |
| 7 |
Cotterall, Cara S (10)
|
MAACAC |
1:04.83 |
59.27 |
-5.56 |
| 8 |
Morrissey, Gabriella M (10)
|
MAJW |
1:00.05 |
59.74 |
-0.31 |
| 9 |
De, Deveshee (10)
|
MAMSA |
1:02.51 |
1:03.25 |
0.74 |
|
Rigney, Marlea M (10)
|
MAWAC |
54.25 |
NS |
- |