| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Brady, Julia M (10)
|
MAGRA |
46.50 |
43.26 |
-3.24 |
| 2 |
Cotterall, Cara S (10)
|
MAACAC |
45.60 |
44.60 |
-1.00 |
| 2 |
Lofgren, Addison E (10)
|
MAACAC |
47.43 |
44.60 |
-2.83 |
| 4 |
Wegner, Abigail (10)
|
MATRA |
49.71 |
46.92 |
-2.79 |
| 5 |
Steinkirchner, Kaitlin P (10)
|
MACYA |
48.11 |
47.24 |
-0.87 |
| 6 |
Stefanelli, Isabella L (10)
|
MAUN-0 |
55.61 |
47.98 |
-7.63 |
| 7 |
Barnett, Emily R (10)
|
MADST |
49.31 |
48.55 |
-0.76 |
| 8 |
Coakley, Sloane V (10)
|
MANLAC |
47.92 |
49.44 |
1.52 |
| 9 |
Chudamani, Avni (10)
|
MAUN-0 |
47.49 |
49.71 |
2.22 |
| 10 |
Teleo, Anysha T (10)
|
MASAC |
52.21 |
50.13 |
-2.08 |
| 11 |
De, Deveshee (10)
|
MAMSA |
52.34 |
50.91 |
-1.43 |
| 12 |
Kulon, Matylda N (10)
|
MADST |
50.95 |
52.23 |
1.28 |
| 13 |
Morrissey, Gabriella M (10)
|
MAJW |
54.17 |
55.05 |
0.88 |