| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Shallow, Megan C (14)
|
MAJW |
2:19.90 |
2:24.90 |
5.00 |
| 2 |
Schneider, Mackenzie L (14)
|
MAJW |
2:29.60 |
2:25.87 |
-3.73 |
| 3 |
Noldy, Jessie (14)
|
MAUN-0 |
2:29.16 |
2:26.61 |
-2.55 |
| 4 |
Staley, Ana M (14)
|
MAKA |
2:32.83 |
2:28.46 |
-4.37 |
| 5 |
Mains-Ferrier, Madison M (14)
|
MAPAAC |
2:31.47 |
2:28.96 |
-2.51 |
| 6 |
Kulon, Claudia (14)
|
MADST |
2:35.33 |
2:31.48 |
-3.85 |
| 7 |
Hegedus, Maggie E (14)
|
MANLAC |
2:49.96 |
2:42.29 |
-7.67 |
| 8 |
Straub, Cassandra J (14)
|
MAJW |
2:50.80 |
2:48.04 |
-2.76 |
|
Bresch, Grace A (14)
|
MAMSC |
2:22.08 |
NS |
- |
|
Bishop, Ayreka A (14)
|
MASAC |
2:45.15 |
NS |
- |