| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kennedy, Delaney S (14)
|
AMANSC |
5:16.35 |
5:07.47 |
-8.88 |
| 2 |
Anderson, Ariana A (13)
|
AMPRA |
5:19.25 |
5:14.60 |
-4.65 |
| 3 |
O'Connor, Brooklyn E (13)
|
AMRXA |
5:19.14 |
5:14.80 |
-4.34 |
| 4 |
Dickson, Eleah A (14)
|
AMPRA |
5:59.10 |
5:15.95 |
-43.15 |
| 5 |
Medjimorec, Megan E (14)
|
AMPRA |
6:32.31 |
5:17.59 |
-74.72 |
| 6 |
Huwar, Sophia G (14)
|
AMANSC |
5:32.16 |
5:27.63 |
-4.53 |
| 7 |
Li, Vivien Y (14)
|
AMANSC |
5:30.63 |
5:28.23 |
-2.40 |
| 8 |
Kile, Fernanda F (13)
|
AMPEAQ |
5:46.76 |
5:28.91 |
-17.85 |
| 9 |
Kass, Savanna L (13)
|
AMRXA |
5:44.64 |
5:37.51 |
-7.13 |
| 10 |
Milliron, Marissa J (14)
|
AMANSC |
6:34.29 |
5:42.60 |
-51.69 |
| 11 |
Coleman, Cassandra J (13)
|
AMPEAQ |
5:43.13 |
5:44.48 |
1.35 |
| 12 |
Anderson, Braleigh A (14)
|
AMBPR |
6:01.16 |
5:49.52 |
-11.64 |