| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cao, Emma M (14)
|
NCTAC |
58.33 |
59.30 |
0.97 |
| 2 |
Jacobs, Audrey L (16)
|
NCPAST |
56.81 |
59.68 |
2.87 |
| 3 |
Nordstrom, Abigail E (15)
|
NCTAC |
59.86 |
1:00.51 |
0.65 |
| 4 |
Flanagan, Emma R (13)
|
NCNEAL |
1:00.66 |
1:02.52 |
1.86 |
| 5 |
Ouellette, Mia G (13)
|
NCGFSC |
1:01.46 |
1:02.89 |
1.43 |
| 6 |
Cyran, Riley M (14)
|
NCTAC |
1:07.84 |
1:04.36 |
-3.48 |
| 7 |
Manners, Ava J (11)
|
NCGFSC |
1:07.36 |
1:05.94 |
-1.42 |
| 8 |
Allen, Riley E (15)
|
NCTAC |
1:05.95 |
1:06.07 |
0.12 |
| 9 |
Ciancarelli, Sophia C (16)
|
NCTAC |
1:11.69 |
1:09.75 |
-1.94 |
| 10 |
Holfelder, Shaye A (14)
|
NCTAC |
1:11.37 |
1:10.79 |
-0.58 |
| 11 |
Kern, Paige L (12)
|
NCPAST |
1:13.62 |
1:10.92 |
-2.70 |
| 12 |
Heise, Ashlin D (14)
|
NCLTNC |
1:15.18 |
1:11.84 |
-3.34 |
| 13 |
Takahashi Goni, Yuriko S (16)
|
NCTAC |
1:11.34 |
1:12.82 |
1.48 |
| 14 |
Suphavadeprasit, Alisha (14)
|
NCTAC |
1:15.08 |
1:14.47 |
-0.61 |
| 15 |
Wilkerson, Olivia S (14)
|
NCGFSC |
1:13.61 |
1:16.91 |
3.30 |
| 16 |
Greaves, London C (12)
|
NCNEAL |
1:25.45 |
1:17.32 |
-8.13 |
| 17 |
Williams, Lauren E (15)
|
NCTAC |
1:17.23 |
1:18.52 |
1.29 |
| 18 |
Tinnin, Anniston M (13)
|
NCPAST |
|
1:25.47 |
- |
| 19 |
Coleman, Rori K (11)
|
NCPAST |
1:40.85 |
1:32.72 |
-8.13 |
| 20 |
Tinnin, Alexis H (11)
|
NCPAST |
|
1:39.66 |
- |
| 21 |
Chriscoe, Sydney R (11)
|
NCNEAL |
1:52.11 |
1:48.87 |
-3.24 |
| 22 |
Trout, Madeline M (11)
|
NCTAC |
1:46.79 |
1:57.93 |
11.14 |