| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Salpeter, Troy M (8)
|
VADIG |
39.22 |
38.74 |
-0.48 |
| 2 |
Bettie, Jr., Kevin M (8)
|
MIDRST |
44.95 |
43.94 |
-1.01 |
| 3 |
Cason, Musaa B (8)
|
MDBCSC |
44.45 |
45.74 |
1.29 |
| 4 |
Smith, Marcos J (8)
|
PVDMV |
45.19 |
47.97 |
2.78 |
| 6 |
Thompson, Elijah J (8)
|
PVDMV |
49.28 |
49.26 |
-0.02 |
| 8 |
Whalen, Eamon M (7)
|
MDMETR |
|
51.02 |
- |
| 9 |
Dorsett, Micah D (8)
|
PVDMV |
51.32 |
51.43 |
0.11 |
| 10 |
Smith, Austin W (7)
|
MDBCSC |
55.98 |
53.17 |
-2.81 |
| 11 |
Zeren, Benjamin P (8)
|
NCRSA |
56.92 |
53.57 |
-3.35 |
| 12 |
Cason, Syeed L (6)
|
CATRA |
55.49 |
54.03 |
-1.46 |
| 13 |
Winstead, Amir J (8)
|
MDYRAC |
56.65 |
54.21 |
-2.44 |
| 18 |
McClam, Marion C (6)
|
NCYSST |
1:06.10 |
59.66 |
-6.44 |
| 19 |
Neff, James M (7)
|
MDBCSC |
55.88 |
59.96 |
4.08 |
| 22 |
Dukes-Robinson, Christopher (8)
|
MDBCSC |
|
1:07.15 |
- |
| 23 |
Harris, Anthony P (6)
|
MIDRST |
1:12.72 |
1:10.40 |
-2.32 |
| 27 |
Cason, Daawood (6)
|
MDBCSC |
1:17.30 |
1:20.95 |
3.65 |
| 28 |
Land, Ethan D (6)
|
MDBCSC |
1:10.21 |
1:20.96 |
10.75 |
|
Cabbagestalk Jr., Travone J (8)
|
MDYRAC |
55.39 |
NS |
- |
|
Ruiz Torres, Angel A (6)
|
PVDMV |
|
NS |
- |