| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Clough, Colin T (12)
|
MAHAC |
26.59 |
26.19 |
-0.40 |
| 2 |
Musante, Benjamin D (12)
|
MAHAC |
27.14 |
26.98 |
-0.16 |
| 3 |
Moll, James M (12)
|
MALAC |
28.84 |
28.06 |
-0.78 |
| 4 |
Brace, Shane L (12)
|
MAHAC |
29.05 |
29.03 |
-0.02 |
| 5 |
Sempsey, Connor (12)
|
MALAC |
33.36 |
30.56 |
-2.80 |
| 6 |
Martin, Nicholas J (12)
|
MAHAC |
31.02 |
30.85 |
-0.17 |
| 7 |
Verma, Aayush (12)
|
MAHAC |
34.47 |
31.03 |
-3.44 |
| 8 |
Swartz, Ethan C (12)
|
MACYA |
32.36 |
31.44 |
-0.92 |
| 9 |
Heisey, Richard J (12)
|
MALAC |
33.75 |
33.17 |
-0.58 |
| 10 |
Hendershot, Carter R (11)
|
MACYA |
33.71 |
33.29 |
-0.42 |
| 11 |
Fetters, Aiden R (11)
|
MACYA |
35.70 |
34.24 |
-1.46 |
| 12 |
Griffith, Charlie E (12)
|
MAHAC |
35.43 |
34.58 |
-0.85 |
| 13 |
Eckert, Jason S (12)
|
MACYA |
37.18 |
35.17 |
-2.01 |
| 14 |
Buckwalter, Darius H (11)
|
MALAC |
36.01 |
36.71 |
0.70 |
| 15 |
Eckert, Jevin (11)
|
MACYA |
41.16 |
40.09 |
-1.07 |