| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wolk, Isabel H (12)
|
NCNCAC |
31.61 |
32.18 |
0.57 |
| 2 |
Emrich, Abigail G (12)
|
NCNCAC |
35.74 |
36.12 |
0.38 |
| 3 |
Kim, Kenzie A (11)
|
NCNCAC |
36.03 |
36.62 |
0.59 |
| 4 |
Shuping, Avery E (12)
|
NCNCAC |
39.95 |
38.06 |
-1.89 |
| 5 |
Giovanello, Aurora J (12)
|
NCNCAC |
37.93 |
38.27 |
0.34 |
| 6 |
Thomas, Arianna M (11)
|
NCNCAC |
37.59 |
38.34 |
0.75 |
| 7 |
Collins, Mackenzie K (11)
|
NCNCAC |
42.43 |
39.56 |
-2.87 |
| 8 |
Taub, Anna J (12)
|
NCNCAC |
40.73 |
41.37 |
0.64 |
| 9 |
Burton, Chloe G (11)
|
NCNCAC |
43.74 |
43.75 |
0.01 |
| 10 |
Li, Joie N (11)
|
NCNCAC |
44.47 |
44.29 |
-0.18 |
| 11 |
Dever, Emerson B (12)
|
NCNCAC |
55.82 |
46.41 |
-9.41 |
| 12 |
Milsom, Molly M (12)
|
NCNCAC |
44.55 |
47.71 |
3.16 |
| 13 |
Yao, Cana (11)
|
NCNCAC |
50.99 |
48.29 |
-2.70 |
| 14 |
Lee, Isabelle A (11)
|
NCNCAC |
55.99 |
51.22 |
-4.77 |
| 15 |
Li, Angela (11)
|
NCNCAC |
48.78 |
51.35 |
2.57 |
| 16 |
Brav, Sapir (12)
|
NCNCAC |
53.26 |
53.75 |
0.49 |
|
Wang, Rui (11)
|
NCNCAC |
1:00.99 |
DQ |
- |