| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Li, Ang (16)
|
NCNCAC |
47.84 |
47.67 |
-0.17 |
| 2 |
Van Vooren, Conner J (17)
|
NCNCAC |
48.44 |
49.08 |
0.64 |
| 3 |
Wohl, Daniel T (17)
|
NCNCAC |
48.29 |
49.24 |
0.95 |
| 4 |
Ren, Jordan S (16)
|
NCNCAC |
48.60 |
50.15 |
1.55 |
| 5 |
Bretzmann, Thomas J (16)
|
NCNCAC |
49.43 |
50.55 |
1.12 |
| 6 |
Hollis, Brian W (15)
|
NCNCAC |
51.16 |
50.65 |
-0.51 |
| 7 |
Foulkes, T'Lez E (15)
|
NCNCAC |
49.68 |
51.10 |
1.42 |
| 8 |
Wickman, Carson R (15)
|
NCNCAC |
51.53 |
51.70 |
0.17 |
| 9 |
Fischer, Christian H (17)
|
NCNCAC |
50.04 |
52.20 |
2.16 |
| 10 |
Brauer, Beau T (17)
|
NCNCAC |
52.42 |
52.84 |
0.42 |
| 11 |
Demko, Jonathan C (16)
|
NCNCAC |
49.42 |
52.88 |
3.46 |
| 12 |
Charboneau, Duncan T (15)
|
NCNCAC |
53.51 |
53.24 |
-0.27 |
| 13 |
Lloyd, Justin C (17)
|
NCNCAC |
51.24 |
53.45 |
2.21 |
| 14 |
Zheng, Andrew (15)
|
NCNCAC |
50.82 |
53.52 |
2.70 |
| 15 |
Wurster, Lincoln M (15)
|
NCNCAC |
52.84 |
54.00 |
1.16 |
| 16 |
Lloyd, Kenneth O (15)
|
NCNCAC |
53.63 |
55.14 |
1.51 |
| 17 |
Grizzle, Samuel J (16)
|
NCNCAC |
56.96 |
57.20 |
0.24 |
| 18 |
Seelbach, Zachary R (15)
|
NCNCAC |
58.86 |
58.58 |
-0.28 |
| 19 |
Greeley, Matthew J (15)
|
NCNCAC |
1:00.52 |
1:01.76 |
1.24 |
| 20 |
Navaratnam, Krishna C (17)
|
NCNCAC |
1:00.54 |
1:02.72 |
2.18 |
| 21 |
Chatterjee, Adiv K (15)
|
NCNCAC |
1:04.41 |
1:05.44 |
1.03 |
|
Samuelson, Seth A (16)
|
NCNCAC |
54.68 |
NS |
- |
|
von Bernuth, Jesko (15)
|
NCNCAC |
|
NS |
- |