| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wolk, Isabel H (12)
|
NCNCAC |
30.82 |
29.78 |
-1.04 |
| 2 |
Shuping, Avery E (12)
|
NCNCAC |
32.09 |
31.16 |
-0.93 |
| 3 |
Emrich, Abigail G (12)
|
NCNCAC |
32.23 |
31.48 |
-0.75 |
| 4 |
Collins, Mackenzie K (11)
|
NCNCAC |
32.39 |
32.03 |
-0.36 |
| 5 |
Giovanello, Aurora J (12)
|
NCNCAC |
36.76 |
35.73 |
-1.03 |
| 6 |
Thomas, Arianna M (11)
|
NCNCAC |
36.76 |
36.40 |
-0.36 |
| 7 |
Kim, Kenzie A (11)
|
NCNCAC |
38.66 |
37.34 |
-1.32 |
| 8 |
Dever, Emerson B (12)
|
NCNCAC |
40.83 |
37.62 |
-3.21 |
| 9 |
Burton, Chloe G (11)
|
NCNCAC |
41.59 |
38.61 |
-2.98 |
| 10 |
Taub, Anna J (12)
|
NCNCAC |
42.42 |
38.79 |
-3.63 |
| 11 |
Li, Joie N (11)
|
NCNCAC |
41.09 |
39.10 |
-1.99 |
| 12 |
Milsom, Molly M (12)
|
NCNCAC |
39.19 |
39.42 |
0.23 |
| 13 |
Yao, Cana (11)
|
NCNCAC |
44.84 |
42.78 |
-2.06 |
| 14 |
Lee, Isabelle A (11)
|
NCNCAC |
50.99 |
44.93 |
-6.06 |
| 15 |
Brav, Sapir (12)
|
NCNCAC |
47.72 |
49.33 |
1.61 |
| 16 |
Li, Angela (11)
|
NCNCAC |
43.74 |
50.56 |
6.82 |
|
Wang, Rui (11)
|
NCNCAC |
55.99 |
DQ |
- |