| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Marsh, Alyssa J (19)
|
NCDUKE |
26.88 |
26.73 |
-0.15 |
| 2 |
Burchill, Veroinca K (19)
|
GAABSC |
27.30 |
27.12 |
-0.18 |
| 3 |
Lindner, Sophie M (17)
|
NCMAC |
27.72 |
27.22 |
-0.50 |
| 4 |
Britt, Chelsea E (22)
|
GAABSC |
27.96 |
27.90 |
-0.06 |
| 5 |
Branaman, Mairyn G (20)
|
SCUN-0 |
|
28.13 |
- |
| 6 |
Stewart, Kylie N (21)
|
GAABSC |
|
28.28 |
- |
| 7 |
Margalis, Melanie A (25)
|
FLSPA |
|
28.58 |
- |
| 8 |
Menkhaus, Julia E (17)
|
NCMAC |
|
28.59 |
- |
| 9 |
Rhodes, Lauren E (21)
|
NCUN-2 |
|
28.69 |
- |
| 10 |
Larochelle, Susanna E (18)
|
NCNCAC |
|
28.78 |
- |
| 11 |
Hylinski, Charlotte A (18)
|
NCMAC |
|
28.82 |
- |
| 12 |
Corbi, Katie M (16)
|
NCMAC |
|
28.93 |
- |
| 12 |
Vannote, Elizabeth A (15)
|
NCMAC |
|
28.93 |
- |
| 14 |
Lowe, Heidi D (17)
|
NCMAC |
|
29.00 |
- |
| 15 |
Harris, Chloe E (15)
|
NCMAC |
|
29.24 |
- |
| 16 |
Cardwell, Mary C (18)
|
GAABSC |
|
29.60 |
- |