| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Smoliga, Olivia M (22)
|
GAABSC |
25.58 |
25.19 |
-0.39 |
| 2 |
Caldwell, Courtney K (19)
|
NCWOLF |
25.70 |
25.24 |
-0.46 |
| 3 |
Cooper, Caitlin M (21)
|
VACAV |
25.86 |
25.57 |
-0.29 |
| 4 |
Burchill, Veroinca K (19)
|
GAABSC |
25.71 |
25.86 |
0.15 |
| 5 |
Kennedy, Madison J (29)
|
NCMAC |
|
25.22 |
- |
| 6 |
Murez, Andrea (25)
|
ZZISR |
|
25.89 |
- |
| 7 |
Marsh, Alyssa J (19)
|
NCDUKE |
|
26.10 |
- |
| 8 |
Ford, Kathryn A (21)
|
NCNCAC |
|
26.51 |
- |
| 8 |
Margalis, Melanie A (25)
|
FLSPA |
|
26.74 |
- |
| 9 |
Woody, Julie J (23)
|
GASST |
|
26.32 |
- |
| 10 |
Fields, Zhada L (19)
|
NCNCAC |
|
26.46 |
- |
| 11 |
Perry, Ky-lee L (18)
|
NCWOLF |
|
26.47 |
- |
| 12 |
Rhodes, Lauren E (21)
|
NCUN-2 |
|
26.58 |
- |
| 13 |
Hess, Madeline M (20)
|
NCDUKE |
|
26.67 |
- |
| 14 |
Stout, Jordan M (19)
|
GAABSC |
|
26.79 |
- |
| 15 |
Flickinger, Madelyn L (15)
|
NCMAC |
|
26.99 |
- |