| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Pugh, Lauren P (11)
|
NCQCD |
41.82 |
40.65 |
-1.17 |
| 2 |
Gause, Kirsten E (12)
|
NCQCD |
44.70 |
42.46 |
-2.24 |
| 3 |
Kramb, Claire E (11)
|
NCTEAM |
46.87 |
44.53 |
-2.34 |
| 4 |
Matvey, Margaret J (12)
|
NCTEAM |
44.54 |
45.79 |
1.25 |
| 5 |
Ferreira, Izabella C (11)
|
NCTEAM |
49.25 |
47.12 |
-2.13 |
| 6 |
Boyd, Sierra E (10)
|
NCTEAM |
|
47.56 |
- |
| 7 |
Rojas, Anne M (10)
|
NCTEAM |
49.23 |
47.94 |
-1.29 |
| 8 |
Picard, Avery E (10)
|
NCTEAM |
52.98 |
48.05 |
-4.93 |
| 9 |
McFadden, Emma M (11)
|
NCQCD |
54.57 |
50.37 |
-4.20 |
| 10 |
McElroy, Zoe N (12)
|
NCQCD |
53.53 |
50.97 |
-2.56 |
| 11 |
Snyder, Elizabeth A (12)
|
NCTEAM |
|
51.88 |
- |
| 12 |
Eickenhorst, Madyn E (10)
|
NCTEAM |
51.84 |
52.12 |
0.28 |
| 13 |
Green, Eva D (9)
|
NCQCD |
1:00.18 |
53.05 |
-7.13 |
| 14 |
Brown, Brooklyn D (11)
|
NCQCD |
54.76 |
53.93 |
-0.83 |
| 15 |
Jimenez-Willis, Evelyn N (11)
|
NCTEAM |
57.88 |
54.89 |
-2.99 |
|
Jones, Myah L (11)
|
NCQCD |
|
DQ |
- |
|
Hunter, Jaliyah D (9)
|
NCQCD |
|
DQ |
- |
|
Golphin, Gabrielle D (12)
|
NCQCD |
|
DQ |
- |
|
Richards, Brady L (12)
|
NCTEAM |
42.17 |
DQ |
- |
|
Davis, Lili G (7)
|
NCQCD |
|
DQ |
- |
|
Hemans, Mackenzie C (10)
|
NCQCD |
|
NS |
- |
|
Sachar, Emma G (11)
|
NCTEAM |
56.18 |
NS |
- |