| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Magner, Caragh M (10)
|
NCMAC |
38.79 |
37.52 |
-1.27 |
| 2 |
Nair, Navami K (10)
|
NCMAC |
41.02 |
43.23 |
2.21 |
| 3 |
Light, Madelyn C (10)
|
NCMAC |
43.42 |
44.49 |
1.07 |
| 4 |
Cankurtaran, Alara M (10)
|
NCMAC |
49.37 |
44.60 |
-4.77 |
| 5 |
Yampolsky, Skye A (10)
|
NCMAC |
45.06 |
44.98 |
-0.08 |
| 6 |
Daniell, Kaylee F (10)
|
NCMAC |
50.81 |
46.37 |
-4.44 |
| 7 |
Royal, Emilia I (10)
|
NCMAC |
55.43 |
47.32 |
-8.11 |
| 8 |
Dickison, Lauren T (10)
|
NCLTNC |
|
48.16 |
- |
| 9 |
Titherington, Anna M (10)
|
NCLTNC |
|
48.25 |
- |
| 10 |
Bahr, Sophia K (10)
|
NCMAC |
48.82 |
48.51 |
-0.31 |
| 11 |
Landrum, Aubrey K (10)
|
NCMAC |
|
50.16 |
- |
| 12 |
Smith, Karlin G (10)
|
NCMAC |
49.32 |
50.55 |
1.23 |
| 13 |
Mohapatra, Anika (10)
|
NCMAC |
|
50.89 |
- |
| 14 |
Lynch, Berkeley C (10)
|
NCMAC |
|
58.91 |
- |
| 15 |
Autrey, Margaret C (10)
|
NCMAC |
|
1:02.54 |
- |
| 16 |
Akula, Pragathi (10)
|
NCMAC |
|
1:03.31 |
- |
| 17 |
Rounds, Jadan B (10)
|
NCMAC |
|
1:05.05 |
- |
|
Lumbanbatu Solano, Andrea G (10)
|
NCMAC |
|
DQ |
- |