| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Pulver, Ayla D (11)
|
NCATOM |
40.08 |
38.84 |
-1.24 |
| 2 |
Li, Stephanie C (12)
|
NCMAC |
40.91 |
39.07 |
-1.84 |
| 3 |
Brandt, Katherine A (12)
|
NCATOM |
41.16 |
41.64 |
0.48 |
| 4 |
Park, Joy J (11)
|
NCMAC |
47.64 |
42.04 |
-5.60 |
| 5 |
Phipps, Harper M (11)
|
NCMAC |
44.92 |
42.76 |
-2.16 |
| 6 |
Anderson, Mikayla L (11)
|
NCMAC |
45.16 |
43.00 |
-2.16 |
| 7 |
Scharsu, Abigail G (11)
|
NCMAC |
42.33 |
43.38 |
1.05 |
| 8 |
Ferrari, Josephine A (11)
|
NCMAC |
44.10 |
43.49 |
-0.61 |
| 9 |
Argenta, Amalia A (11)
|
NCMAC |
45.71 |
43.51 |
-2.20 |
| 10 |
Mann, Phoebe M (11)
|
NCMAC |
45.26 |
43.61 |
-1.65 |
| 11 |
Garsik, Addison M (11)
|
NCUN |
44.72 |
44.05 |
-0.67 |
| 12 |
Lynch, Berkeley C (12)
|
NCMAC |
47.14 |
45.25 |
-1.89 |
| 13 |
Wiley, Cacey A (12)
|
NCMAC |
45.81 |
45.45 |
-0.36 |
| 14 |
Lumbanbatu Solano, Andrea G (11)
|
NCMAC |
49.20 |
47.32 |
-1.88 |
| 15 |
Nardone, Avery Y (12)
|
NCMAC |
49.89 |
47.70 |
-2.19 |
| 16 |
Brown, Mary G (11)
|
NCMAC |
51.10 |
47.72 |
-3.38 |
| 17 |
Rounds, Jadan B (11)
|
NCMAC |
50.24 |
48.02 |
-2.22 |
| 18 |
Gilmore, Rileigh P (11)
|
NCMAC |
50.86 |
50.45 |
-0.41 |
| 19 |
Mahoney, Katie A (11)
|
NCMAC |
55.93 |
50.47 |
-5.46 |
| 20 |
Akula, Pragathi (11)
|
NCMAC |
56.01 |
50.87 |
-5.14 |
| 21 |
McDaniel, Katherine T (11)
|
NCMAC |
|
51.04 |
- |
| 22 |
FitzPatrick, Ava M (11)
|
NCMAC |
54.13 |
51.14 |
-2.99 |
| 23 |
Cole, Madelyn A (11)
|
NCATOM |
56.20 |
53.06 |
-3.14 |
| 24 |
Krupinski, Harlow M (12)
|
NCMAC |
57.40 |
53.65 |
-3.75 |
| 25 |
Miano, Olivia W (11)
|
NCMAC |
55.59 |
53.78 |
-1.81 |
| 26 |
Freeland, Mikayla R (11)
|
NCATOM |
|
58.12 |
- |
|
Pagano, Emily J (12)
|
NCMAC |
1:06.07 |
NS |
- |
|
Baker, Avery A (12)
|
NCMAC |
41.01 |
NS |
- |