| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Yampolsky, Skye A (10)
|
NCMAC |
|
47.12 |
- |
| 2 |
Scharsu, Abigail G (10)
|
NCMAC |
55.67 |
51.50 |
-4.17 |
| 3 |
Bass, Lillian C (10)
|
NCMAC |
56.19 |
52.26 |
-3.93 |
| 4 |
Bahr, Sophia K (10)
|
NCMAC |
59.98 |
53.65 |
-6.33 |
| 5 |
Connors, Emily R (10)
|
NCMAC |
56.11 |
54.10 |
-2.01 |
| 6 |
Holden, Ainsley M (10)
|
NCMAC |
1:00.09 |
54.93 |
-5.16 |
| 7 |
Eller, Kendall E (10)
|
NCMAC |
57.55 |
55.49 |
-2.06 |
| 8 |
Wickerham, Catherine R (10)
|
NCMAC |
|
55.73 |
- |
| 9 |
O'Dell, Sarah G (10)
|
NCMAC |
59.67 |
56.03 |
-3.64 |
| 10 |
Galvin, Prema R (10)
|
NCMAC |
57.05 |
56.52 |
-0.53 |
| 11 |
Claudy, Julia A (10)
|
NCMAC |
54.65 |
56.70 |
2.05 |
| 12 |
Royal, Emilia I (10)
|
NCMAC |
1:01.63 |
59.21 |
-2.42 |
| 13 |
Lindsay, Ann M (10)
|
NCMAC |
58.47 |
59.94 |
1.47 |
| 14 |
Martin, Avery G (10)
|
NCUN |
|
1:01.25 |
- |
| 15 |
Schiffern, Taylor G (10)
|
NCMAC |
1:07.75 |
1:04.81 |
-2.94 |
| 16 |
Rounds, Jadan B (10)
|
NCMAC |
|
1:04.89 |
- |
| 17 |
Daniell, Kaylee F (10)
|
NCMAC |
1:05.79 |
1:05.41 |
-0.38 |
| 18 |
Lumbanbatu Solano, Andrea G (10)
|
NCMAC |
|
1:05.64 |
- |
| 19 |
Miano, Olivia W (10)
|
NCMAC |
1:13.86 |
1:06.57 |
-7.29 |