| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kudela, Caroline R (14)
|
NCMSA |
4:54.55 |
4:47.47 |
-7.08 |
| 2 |
Greco, Mary G (18)
|
NCMSA |
4:47.54 |
4:51.54 |
4.00 |
| 3 |
Lee, Emma K (12)
|
NCMSA |
5:06.45 |
5:00.52 |
-5.93 |
| 4 |
Hamblin, Kaylee J (15)
|
NCMSA |
5:01.23 |
5:12.61 |
11.38 |
| 5 |
Bernesser, Skylar H (16)
|
NCMSA |
5:00.79 |
5:21.57 |
20.78 |
| 6 |
Ku, Katherine E (16)
|
NCTEAM |
5:45.99 |
5:21.95 |
-24.04 |
| 7 |
Holshouser, Maci R (14)
|
NCSAIL |
5:56.75 |
5:27.53 |
-29.22 |
| 8 |
Volz, Julia R (19)
|
NCSAIL |
5:21.03 |
5:28.99 |
7.96 |
| 9 |
Altmeyer, Lillian K (14)
|
NCTEAM |
5:15.10 |
5:30.46 |
15.36 |
| 10 |
Jaynes, Allyson M (16)
|
NCSAIL |
5:13.24 |
5:34.36 |
21.12 |
| 11 |
Linkel, Lorelei G (14)
|
NCSAIL |
5:48.21 |
5:37.31 |
-10.90 |
| 12 |
Pratt, Morgan E (14)
|
NCSAIL |
5:35.82 |
5:38.91 |
3.09 |
| 13 |
Horton, Presley C (13)
|
NCSAIL |
|
5:43.69 |
- |
| 14 |
Phillips, Abigail N (15)
|
NCSAIL |
5:50.12 |
5:52.03 |
1.91 |
| 15 |
Gillenwater, Gretchen R (15)
|
NCNSS |
6:04.00 |
5:53.76 |
-10.24 |
| 16 |
Donahue, Gracelyn D (13)
|
NCSAIL |
|
6:02.20 |
- |
| 17 |
Carlton, Kendall A (13)
|
NCSAIL |
|
6:07.12 |
- |
| 18 |
Newhouse, Madeline R (13)
|
NCSAIL |
|
6:12.43 |
- |
| 19 |
Carney, Kaitlyn C (13)
|
NCMSA |
8:00.00 |
6:14.71 |
-105.29 |
|
Burroughs, Hayden E (13)
|
NCSAIL |
|
DQ |
- |