| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Slowey, William A (12)
|
SCSCSC |
34.44 |
33.69 |
-0.75 |
| 2 |
Guenther, Jack C (12)
|
NCATOM |
34.55 |
34.25 |
-0.30 |
| 3 |
McKinney, Ian M (12)
|
NCMSA |
40.09 |
35.66 |
-4.43 |
| 4 |
Deng, Jake (12)
|
NCATOM |
35.11 |
35.72 |
0.61 |
| 5 |
Hanks, Wyatt L (12)
|
NCUN |
43.09 |
35.98 |
-7.11 |
| 6 |
Auman, Isaiah H (12)
|
NCEAC |
36.62 |
36.45 |
-0.17 |
| 7 |
Grimm, Gabriel R (11)
|
SCSCSC |
36.60 |
36.51 |
-0.09 |
| 8 |
Bangoura, Gabriel S (11)
|
NCTEAM |
35.94 |
36.68 |
0.74 |
| 9 |
Sulkowski, Matthew A (12)
|
SCSCSC |
39.13 |
36.82 |
-2.31 |
| 10 |
Bowen, Jackson D (12)
|
GAOAC |
38.83 |
39.07 |
0.24 |
| 11 |
Gates, Tilman T (12)
|
NCNSS |
39.77 |
39.50 |
-0.27 |
| 12 |
Herger, Paul C (12)
|
NCATOM |
39.81 |
39.75 |
-0.06 |
| 13 |
Hills, Jacob S (11)
|
SCSCSC |
41.67 |
40.38 |
-1.29 |
| 14 |
Bryson, Rutherford C (12)
|
NCATOM |
41.55 |
42.34 |
0.79 |
|
Norris, Andrew R (11)
|
SCSCSC |
35.34 |
NS |
- |