| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Eward, Ellery L (10)
|
NCNCAC |
2:28.22 |
2:26.05 |
-2.17 |
| 2 |
Emrich, Abigail G (10)
|
NCNCAC |
2:36.86 |
2:26.47 |
-10.39 |
| 3 |
Boyette, Sydney H (9)
|
SCSCSC |
2:48.27 |
2:30.47 |
-17.80 |
| 4 |
Wirz, Valerie G (10)
|
NCNSS |
2:35.84 |
2:31.19 |
-4.65 |
| 5 |
Shuping, Avery E (10)
|
NCNCAC |
2:37.81 |
2:37.56 |
-0.25 |
| 6 |
Wiggins, Harper M (10)
|
NCNCAC |
2:50.40 |
2:41.97 |
-8.43 |
| 7 |
Ferreira, Izabella C (10)
|
NCTEAM |
2:53.48 |
2:44.04 |
-9.44 |
| 8 |
Rojas, Anne M (9)
|
NCTEAM |
2:51.31 |
2:47.91 |
-3.40 |
| 9 |
Thomas, Arianna M (10)
|
NCNCAC |
3:08.96 |
2:48.60 |
-20.36 |
| 10 |
Giovanello, Aurora J (10)
|
NCNCAC |
2:45.94 |
2:49.79 |
3.85 |
| 11 |
Norris, Madison R (9)
|
NCTEAM |
2:54.34 |
2:51.28 |
-3.06 |
| 12 |
Luo, Grace L (10)
|
NCNCAC |
3:28.18 |
2:52.86 |
-35.32 |
| 13 |
Picard, Avery E (9)
|
NCUNTE |
2:55.55 |
2:57.37 |
1.82 |