| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Nair, Navami K (10)
|
NCMAC |
37.21 |
33.96 |
-3.25 |
| 2 |
Daniell, Kaylee F (10)
|
NCMAC |
37.25 |
34.67 |
-2.58 |
| 3 |
Yampolsky, Skye A (10)
|
NCMAC |
36.33 |
35.11 |
-1.22 |
| 4 |
Smith, Karlin G (10)
|
NCMAC |
39.80 |
36.72 |
-3.08 |
| 5 |
Payne, Savannah K (10)
|
NCMAC |
|
36.92 |
- |
| 6 |
Titherington, Anna M (10)
|
NCLTNC |
|
37.06 |
- |
| 6 |
Pero, Sophia I (10)
|
NCMAC |
37.11 |
37.06 |
-0.05 |
| 8 |
Argenta, Amalia A (10)
|
NCMAC |
38.63 |
37.20 |
-1.43 |
| 9 |
Light, Madelyn C (10)
|
NCMAC |
39.04 |
37.42 |
-1.62 |
| 10 |
Mann, Phoebe M (10)
|
NCMAC |
|
38.23 |
- |
| 11 |
Dickison, Lauren T (10)
|
NCLTNC |
|
38.28 |
- |
| 12 |
Bahr, Sophia K (10)
|
NCMAC |
41.26 |
39.98 |
-1.28 |
| 13 |
Mahoney, Katie A (10)
|
NCMAC |
|
40.82 |
- |
| 14 |
Lumbanbatu Solano, Andrea G (10)
|
NCMAC |
|
41.85 |
- |
| 15 |
Rounds, Jadan B (10)
|
NCMAC |
43.85 |
42.66 |
-1.19 |
| 16 |
Riddle, Taylor M (10)
|
NCMAC |
45.90 |
42.82 |
-3.08 |
| 17 |
Connors, Emily R (10)
|
NCMAC |
40.07 |
43.00 |
2.93 |