| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Thomas, Morgan O (16)
|
VACA-Y |
23.01 |
23.84 |
0.83 |
| 2 |
Yu, Sophia (18)
|
VACA-Y |
23.51 |
25.70 |
2.19 |
| 3 |
Bendall, Elisabeth A (18)
|
VACA-Y |
25.12 |
25.74 |
0.62 |
| 4 |
Mizuba, Hannah R (14)
|
VAVABR |
24.92 |
25.75 |
0.83 |
| 5 |
Fix, Cayden M (16)
|
VACA-Y |
24.64 |
25.77 |
1.13 |
| 6 |
Wells, Madelyn I (17)
|
VACA-Y |
25.18 |
26.76 |
1.58 |
| 7 |
Ma, Sophia (16)
|
VACA-Y |
25.65 |
27.05 |
1.40 |
| 8 |
Kovarik, Katherine A (14)
|
VALY |
27.33 |
27.56 |
0.23 |
| 9 |
Ward, Alena F (14)
|
VAVABR |
27.33 |
27.71 |
0.38 |
| 10 |
Wagoner, Arleigh K (17)
|
VALY |
27.10 |
28.10 |
1.00 |
| 11 |
Marotta, Caroline G (18)
|
VALY |
27.32 |
28.14 |
0.82 |
| 12 |
Wilkes, Carly A (17)
|
VAVABR |
27.89 |
29.10 |
1.21 |
| 13 |
Jablonski, Lily E (16)
|
VALY |
27.52 |
29.25 |
1.73 |
| 14 |
Becks, Jaeanah Z (14)
|
VAVABR |
29.44 |
29.96 |
0.52 |
| 15 |
Gustafson, Zelynn C (13)
|
VALY |
32.35 |
32.97 |
0.62 |