| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Tedeschi, Roman (12)
|
NIAMTS |
32.92 |
32.85 |
-0.07 |
| 2 |
Borden, Jonas O (12)
|
NIBAAC |
33.33 |
34.25 |
0.92 |
| 3 |
Coen, Liam C (11)
|
NISTAR |
36.08 |
36.38 |
0.30 |
| 4 |
Hayter, Daniel J (11)
|
NISHAQ |
36.72 |
37.84 |
1.12 |
| 5 |
LaMoy, Hunter P (12)
|
NIRAYS |
37.46 |
38.19 |
0.73 |
| 6 |
Szmanda, Cristopher D (12)
|
NIRAYS |
37.92 |
39.39 |
1.47 |
| 7 |
Remick, Spencer S (12)
|
NIBAAC |
42.95 |
39.60 |
-3.35 |
| 8 |
Coen, Jack D (11)
|
NISTAR |
37.15 |
40.49 |
3.34 |
| 9 |
Avino, Evan A (12)
|
NISTAR |
42.78 |
42.23 |
-0.55 |
| 10 |
Littrell, Alexander W (12)
|
NISTAR |
42.90 |
43.51 |
0.61 |
| 11 |
Tareen, Andrew J (11)
|
NITTSC |
|
43.90 |
- |
| 12 |
Roche, Robert (11)
|
NISTAR |
43.86 |
46.17 |
2.31 |
|
Gross, Noah R (12)
|
NISTAR |
38.35 |
NS |
- |