| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Priset, Kyle J (12)
|
NICLSC |
|
40.50 |
- |
| 2 |
Coen, Jack D (11)
|
NISTAR |
42.16 |
41.95 |
-0.21 |
| 3 |
Hayter, Daniel J (11)
|
NISHAQ |
41.95 |
42.12 |
0.17 |
| 4 |
Schraufstetter, Evan (12)
|
NITTSC |
45.62 |
43.13 |
-2.49 |
| 5 |
Avino, Evan A (12)
|
NISTAR |
46.35 |
45.60 |
-0.75 |
| 6 |
LaMoy, Hunter P (12)
|
NIRAYS |
48.69 |
49.20 |
0.51 |
| 7 |
Gross, Noah R (11)
|
NISTAR |
54.23 |
50.03 |
-4.20 |
| 8 |
Carpenter, Trenten E (12)
|
NIRAYS |
|
51.83 |
- |
|
Spaeth, Alexander J (12)
|
NISTAR |
|
DQ |
- |
|
Woodford, Derek (11)
|
NISHAQ |
54.05 |
DQ |
- |
|
Tedeschi, Roman (11)
|
NIAMTS |
|
NS |
- |
|
Roche, Robert (11)
|
NISTAR |
56.88 |
NS |
- |