| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cordle, Gracie A (13)
|
NIFALC |
36.47 |
36.34 |
-0.13 |
| 2 |
Chaplin, Allison V (14)
|
NITTSC |
36.17 |
38.49 |
2.32 |
| 3 |
Roberts, Alaina G (13)
|
NITTSC |
42.68 |
39.86 |
-2.82 |
| 4 |
Gauld, Alison M (13)
|
NISHAQ |
41.41 |
41.42 |
0.01 |
| 5 |
Randall, Alexandra E (14)
|
NISHAQ |
42.68 |
41.91 |
-0.77 |
| 6 |
Higley, Kayla P (14)
|
NIRAYS |
43.46 |
43.21 |
-0.25 |
| 7 |
Del Regno, Jaina R (13)
|
NIRAYS |
42.55 |
43.36 |
0.81 |
| 8 |
Calderon, Jennifer A (14)
|
NISTAR |
|
43.44 |
- |
| 9 |
Hauser-Howells, Lillian K (13)
|
NITTSC |
45.96 |
45.66 |
-0.30 |
| 10 |
Hall, Jenna E (13)
|
NIWWSC |
|
48.20 |
- |
|
Faller, Karsen E (13)
|
NIAMTS |
38.31 |
NS |
- |