| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Roberts, Alaina G (13)
|
NITTSC |
35.68 |
36.12 |
0.44 |
| 2 |
Clouser, Kristiana I (13)
|
NIBCSR |
36.69 |
36.46 |
-0.23 |
| 3 |
Randall, Alexandra E (14)
|
NISHAQ |
37.24 |
36.57 |
-0.67 |
| 4 |
Calderon, Jennifer A (14)
|
NISTAR |
|
36.92 |
- |
| 5 |
Sousis, Gabriella R (14)
|
NISTAR |
38.09 |
37.43 |
-0.66 |
| 6 |
Donlon, Anna M (13)
|
NIWWSC |
37.35 |
38.23 |
0.88 |
| 7 |
Gauld, Alison M (13)
|
NISHAQ |
42.93 |
38.62 |
-4.31 |
| 8 |
Higley, Kayla P (14)
|
NIRAYS |
44.97 |
41.95 |
-3.02 |
| 9 |
Cameron, Nzingha A (13)
|
NIBCSR |
41.55 |
41.98 |
0.43 |
|
Hall, Jenna E (13)
|
NIWWSC |
41.15 |
DQ |
- |