| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wichhart, Benjamin A (12)
|
NIWWSC |
32.53 |
28.78 |
-3.75 |
| 2 |
Faller, Kai P (11)
|
NIAMTS |
29.93 |
29.23 |
-0.70 |
| 3 |
Carpenter, Trenten E (12)
|
NIRAYS |
31.92 |
31.44 |
-0.48 |
| 4 |
Coen, Jack D (11)
|
NISTAR |
35.75 |
33.12 |
-2.63 |
| 5 |
Wittmann, Mark (12)
|
NIAMTS |
34.06 |
33.21 |
-0.85 |
| 6 |
LaMoy, Hunter P (12)
|
NIRAYS |
34.21 |
34.95 |
0.74 |
| 7 |
Gross, Noah R (11)
|
NISTAR |
35.01 |
36.00 |
0.99 |
| 8 |
Szmanda, Cristopher D (11)
|
NIRAYS |
34.85 |
37.15 |
2.30 |
| 9 |
Avino, Evan A (12)
|
NISTAR |
38.62 |
37.35 |
-1.27 |
| 10 |
Lesmeister, Nicholas M (12)
|
NITTSC |
40.20 |
43.33 |
3.13 |
| 11 |
Littrell, Alexander W (12)
|
NISTAR |
43.48 |
43.99 |
0.51 |
| 12 |
Roberts, Harrison F (11)
|
NITTSC |
48.54 |
44.41 |
-4.13 |
| 13 |
Noga, Brendan J (11)
|
NIAMTS |
47.61 |
45.44 |
-2.17 |
| 14 |
Spaeth, Alexander J (12)
|
NISTAR |
48.74 |
49.94 |
1.20 |
|
Gatti, Jake D (12)
|
NICLSC |
32.99 |
NS |
- |
|
Roche, Robert (11)
|
NISTAR |
41.37 |
NS |
- |