| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sedki, Alec (14)
|
NITTSC |
26.03 |
27.01 |
0.98 |
| 2 |
Pike, Clayton B (13)
|
NISTAR |
27.11 |
27.89 |
0.78 |
| 3 |
Farruggia, Michael C (14)
|
NITTSC |
28.93 |
28.35 |
-0.58 |
| 4 |
Weimer, Henry G (13)
|
NICLSC |
29.49 |
28.77 |
-0.72 |
| 5 |
Zynda, Trevor J (13)
|
NIUN-S |
30.28 |
28.78 |
-1.50 |
| 6 |
Jensen, Stuart M (13)
|
NICLSC |
32.41 |
30.19 |
-2.22 |
| 7 |
Cowell, Braden S (13)
|
NISHAQ |
30.08 |
31.38 |
1.30 |
| 8 |
Boglioli, James D (14)
|
NICLSC |
33.97 |
31.47 |
-2.50 |
| 9 |
Renzoni, Nicholas G (13)
|
NISTAR |
34.26 |
31.72 |
-2.54 |
| 10 |
Signore, Christopher A (13)
|
NITTSC |
33.76 |
34.71 |
0.95 |
| 11 |
Gorney, Caleb A (13)
|
NITTSC |
38.78 |
36.24 |
-2.54 |
| 12 |
Glahe, Benjamin S (13)
|
NIWWSC |
44.34 |
38.93 |
-5.41 |