| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mombrea, Joseph R (16)
|
NIWWSC |
23.50 |
22.34 |
-1.16 |
| 2 |
Mixon, Matthew D (18)
|
NIWWSC |
23.80 |
23.46 |
-0.34 |
| 3 |
Miller, Dylan M (17)
|
NIWWSC |
24.40 |
23.48 |
-0.92 |
| 4 |
Clouser, Kameron D (17)
|
NIBCSR |
24.28 |
24.47 |
0.19 |
| 5 |
Maciejewski, Jacob R (16)
|
NIRAYS |
29.08 |
25.45 |
-3.63 |
| 6 |
Crego, Alex N (15)
|
NICLSC |
28.88 |
27.55 |
-1.33 |
| 7 |
Nienhaus, Jack (15)
|
NISHAQ |
28.04 |
28.59 |
0.55 |
| 8 |
Wiegand, Jakob W (15)
|
NITTSC |
28.00 |
28.91 |
0.91 |
| 9 |
Switalski, Nicholas M (15)
|
NITTSC |
28.23 |
29.19 |
0.96 |
| 10 |
LeFever, Nathan M (16)
|
NIRAYS |
31.26 |
30.63 |
-0.63 |
| 11 |
McCall, Akhil J (15)
|
NIBCSR |
32.59 |
31.10 |
-1.49 |