| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hartley, Thea J (7)
|
VATIDE |
30.31 |
28.19 |
-2.12 |
| 2 |
Zmuda, Kimberly N (7)
|
VAECAT |
31.32 |
29.56 |
-1.76 |
| 3 |
Wilkerson, Charlotte A (7)
|
VATIDE |
31.97 |
30.03 |
-1.94 |
| 4 |
Krell, Riley A (7)
|
VAECAT |
27.38 |
30.58 |
3.20 |
| 5 |
Lucas, Sophia G (7)
|
VAECAT |
30.53 |
31.04 |
0.51 |
| 6 |
Wieck, Cailey J (7)
|
VAODAC |
31.28 |
31.68 |
0.40 |
| 7 |
Bonfiglio, Leah T (7)
|
VATIDE |
38.92 |
34.02 |
-4.90 |
| 8 |
Embrey, Chloe F (7)
|
VATIDE |
|
34.63 |
- |
| 9 |
Ryan, Katelyn A (7)
|
VAODAC |
|
40.57 |
- |
| 10 |
Foxworth, Katherine E (7)
|
VAODAC |
41.72 |
40.96 |
-0.76 |
| 11 |
MacDonaugh, Teaghan A (7)
|
VATIDE |
|
1:03.40 |
- |
|
Berdoulay, Ella K (7)
|
VAODAC |
|
DQ |
- |