| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Polhamus, Grace (17)
|
WICVY |
26.10 |
26.82 |
0.72 |
| 2 |
Berg, Sydnea (17)
|
WICVY |
26.05 |
26.90 |
0.85 |
| 3 |
Adams, Rebecca L (17)
|
WICVY |
|
27.23 |
- |
| 4 |
Secker, Callista J (15)
|
WIECY |
27.22 |
28.24 |
1.02 |
| 5 |
Archibald-Smith, Paige G (14)
|
WIECY |
28.08 |
28.79 |
0.71 |
| 6 |
Scholl, Ava (14)
|
WILAXY |
29.05 |
29.58 |
0.53 |
| 7 |
Schneider, Emma K (17)
|
WICVY |
27.52 |
29.70 |
2.18 |
| 8 |
Alden, Alyssa A (15)
|
WITFSC |
29.63 |
30.35 |
0.72 |
| 9 |
Archibald-Smith, Madalyn C (17)
|
WIECY |
29.58 |
30.50 |
0.92 |
| 10 |
Kotnour, Maria G (13)
|
WILAXY |
30.29 |
30.61 |
0.32 |
| 11 |
Beenken, Maje (16)
|
MNTCCS |
30.55 |
30.66 |
0.11 |
| 12 |
Isenberger, Kira (15)
|
WICVY |
33.76 |
30.88 |
-2.88 |
| 13 |
Beltz, Natalie E (15)
|
WIECY |
29.94 |
30.98 |
1.04 |
| 14 |
Heers, Alli S (15)
|
ECAC |
29.91 |
31.07 |
1.16 |
| 15 |
Sirianni, Amber L (15)
|
WIECY |
|
31.48 |
- |
| 16 |
Kinlen, Sophia (16)
|
WICVY |
32.67 |
32.50 |
-0.17 |
| 17 |
Kline, Madeleine E (16)
|
WITFSC |
32.60 |
33.83 |
1.23 |
|
Anderson, Juli Ya (13)
|
WICVY |
38.41 |
NS |
- |