| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Arrowood, Brock (12)
|
WIRSC |
29.54 |
29.75 |
0.21 |
| 2 |
O'Malley, Dolan J (12)
|
WIRSC |
32.61 |
29.90 |
-2.71 |
| 3 |
Denis, Shawn (12)
|
WIRSC |
30.79 |
29.96 |
-0.83 |
| 4 |
Anderson, Elijah (12)
|
WICVY |
32.17 |
31.64 |
-0.53 |
| 5 |
Zwiefelhofer, Ashton (11)
|
WICVY |
41.94 |
32.21 |
-9.73 |
| 6 |
Rogge, Seth (11)
|
WICVY |
32.75 |
33.23 |
0.48 |
| 7 |
Skutley, Benjamin M (12)
|
WIECY |
37.15 |
34.00 |
-3.15 |
| 8 |
Rukavina, Ari J (12)
|
WIBRYS |
36.48 |
35.20 |
-1.28 |
| 9 |
Anderson, Zachary (11)
|
WICVY |
39.46 |
37.71 |
-1.75 |
| 10 |
Larson, Christopher (11)
|
WIRSC |
39.83 |
38.20 |
-1.63 |
| 11 |
Nelson, Caden E (11)
|
WIECY |
36.55 |
38.47 |
1.92 |
| 12 |
Luce, Caleb (11)
|
WIRSC |
39.19 |
39.56 |
0.37 |
| 13 |
Sehgal, Aarav (11)
|
WIECY |
|
39.89 |
- |
| 14 |
Clairmore, Ethan G (11)
|
WIRSC |
|
44.81 |
- |
| 15 |
McBride, Easton (11)
|
WICVY |
52.89 |
47.27 |
-5.62 |
| 16 |
Scott, Tobias L (11)
|
WIRSC |
|
48.06 |
- |
| 17 |
Manjunatha, Pranav N (12)
|
WIECY |
|
51.98 |
- |