| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Helminiak, Lauren M (12)
|
MDMAS |
25.00 |
24.92 |
-0.08 |
| 2 |
Dvorak, Mia I (13)
|
MDACA |
25.40 |
25.49 |
0.09 |
| 2 |
Chadwick, Ellen K (14)
|
PVYORK |
24.92 |
25.49 |
0.57 |
| 4 |
Bothwell, Amelia L (17)
|
MDBAAC |
25.65 |
25.64 |
-0.01 |
| 5 |
Marcum, Ashley M (15)
|
MDHFY |
2:10.75 |
25.88 |
-104.87 |
| 6 |
Koch, Taylor N (17)
|
MDFAST |
25.59 |
26.02 |
0.43 |
| 7 |
Hawes, Tess A (17)
|
MDCAA! |
25.87 |
26.47 |
0.60 |
| 8 |
Edsall, Lauren F (16)
|
MDFAST |
26.40 |
26.86 |
0.46 |
| 9 |
Helminiak, Katherine E (15)
|
MDMAS |
25.23 |
26.90 |
1.67 |
| 10 |
McDaniels, Madison T (14)
|
MDMAS |
26.41 |
26.98 |
0.57 |