| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Morton, Isabella C (8)
|
VAHOKI |
43.81 |
40.25 |
-3.56 |
| 2 |
Jones, Kathryn M (8)
|
VACCA |
40.56 |
40.63 |
0.07 |
| 3 |
Burgess, Maggie J (8)
|
VAQSTS |
40.36 |
41.39 |
1.03 |
| 4 |
Supanich, Isabella J (8)
|
VAVABR |
39.13 |
41.82 |
2.69 |
| 5 |
Athey, Parker K (8)
|
VAGATR |
41.63 |
42.41 |
0.78 |
| 6 |
Hynst, Lexi M (8)
|
VASRVA |
46.81 |
42.74 |
-4.07 |
| 7 |
Williams, Isabelle J (8)
|
VAGATR |
46.51 |
44.63 |
-1.88 |
| 8 |
McKinnon, Christina E (8)
|
VAQSTS |
42.44 |
46.96 |
4.52 |
| 9 |
Oswald, Daylie J (7)
|
VASMAC |
47.16 |
47.02 |
-0.14 |
| 10 |
Rutherford, Abigail H (8)
|
VACA-Y |
52.08 |
49.12 |
-2.96 |
| 11 |
Brooks, Charleston E (6)
|
VAHNVR |
|
54.02 |
- |
| 12 |
Jamme, Mary-Louise P (8)
|
VASMAC |
49.55 |
55.90 |
6.35 |
| 13 |
Moore, Madeline C (6)
|
VAGATR |
59.26 |
58.00 |
-1.26 |
| 14 |
Joyner, Katherine G (7)
|
VAPSDN |
52.93 |
58.34 |
5.41 |
| 15 |
Lewis, Cerys A (8)
|
VAPSDN |
1:11.95 |
59.31 |
-12.64 |
| 16 |
Mancini, Harper Q (7)
|
VAGATR |
1:03.48 |
59.32 |
-4.16 |
| 17 |
Zayas, Paisley E (6)
|
VAGATR |
1:07.62 |
1:12.33 |
4.71 |
| 18 |
Kearns, Tyler S (8)
|
VACCA |
1:26.26 |
1:23.39 |
-2.87 |
|
Knappe, Sara C (7)
|
VACCA |
|
NS |
- |