| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Jones, Layne C (11)
|
VACCA |
|
36.55 |
- |
| 2 |
Nichols, Cassandra L (11)
|
NCRSA |
38.47 |
36.90 |
-1.57 |
| 3 |
Jones, Madison O (11)
|
NCEAC |
37.54 |
37.18 |
-0.36 |
| 4 |
Grim, Evelyn M (11)
|
VAHOKI |
40.96 |
37.19 |
-3.77 |
| 5 |
Wilhite, Charlotte L (11)
|
PVMACH |
41.22 |
38.35 |
-2.87 |
| 6 |
Spivey, Tia F (11)
|
SEBSC |
42.68 |
41.60 |
-1.08 |
| 7 |
Edwards, Natalie M (11)
|
VAHOKI |
|
41.65 |
- |
| 8 |
Jones, Shelby L (11)
|
VAHOKI |
|
41.82 |
- |
| 9 |
Carter, Maddi G (11)
|
VACCA |
48.84 |
42.30 |
-6.54 |
| 10 |
Sturgill, Lydia K (11)
|
PVMACH |
48.28 |
43.38 |
-4.90 |
| 11 |
Bernedo, Helaina S (11)
|
SEBSC |
|
43.66 |
- |
| 12 |
Viar, Annaliese R (11)
|
VACCA |
47.03 |
44.89 |
-2.14 |
| 13 |
Hedrick, Alexandra T (11)
|
NCTEAM |
|
45.26 |
- |
| 14 |
Sachar, Emma G (11)
|
NCTEAM |
|
46.87 |
- |
| 15 |
Fentress, Harmony L (11)
|
VAHOKI |
|
47.43 |
- |
| 16 |
Miller, Deanna R (11)
|
SEBSC |
58.81 |
51.92 |
-6.89 |
| 17 |
Proco, Chloe A (11)
|
NCEAC |
57.47 |
1:01.93 |
4.46 |