| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mizuba, Hannah R (11)
|
VASFY |
36.26 |
36.80 |
0.54 |
| 2 |
Sylvester-Johnson, Jillian B (11)
|
VAGATR |
37.32 |
38.94 |
1.62 |
| 3 |
Krieger, Katelyn N (11)
|
NCEAC |
|
39.15 |
- |
| 4 |
Martin, Hannah C (11)
|
VAHOKI |
38.57 |
39.62 |
1.05 |
| 5 |
Patnoe, Lily G (11)
|
WVYKVK |
38.80 |
39.83 |
1.03 |
| 6 |
Fernando, Scarlett O (11)
|
NCEAC |
39.02 |
39.90 |
0.88 |
| 7 |
Mitias, Ava M (11)
|
WVYKVK |
40.33 |
39.95 |
-0.38 |
| 8 |
Carter, Emmi D (11)
|
VACCA |
42.03 |
40.79 |
-1.24 |
| 9 |
Howards, Nina P (11)
|
NCEAC |
42.20 |
41.76 |
-0.44 |
| 10 |
Murtaugh, Lindsay E (11)
|
VAGATR |
42.44 |
41.89 |
-0.55 |
| 11 |
Byrnes, Jia L (11)
|
VAGATR |
42.52 |
42.68 |
0.16 |
| 12 |
Sanford, Anna M (11)
|
VAHOKI |
43.70 |
43.23 |
-0.47 |
| 13 |
Ferreira, Clara (11)
|
VAHOKI |
48.34 |
47.03 |
-1.31 |
| 14 |
Day, Ella G (11)
|
VAHOKI |
51.48 |
51.08 |
-0.40 |
|
Hansen, Suvie M (11)
|
VACCA |
|
NS |
- |
|
Viar, Olivia B (11)
|
VACCA |
40.23 |
NS |
- |