| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Muzzy, Ava M (12)
|
VAGATR |
34.17 |
33.84 |
-0.33 |
| 2 |
Lusk, Zoey M (12)
|
VAGATR |
34.98 |
33.99 |
-0.99 |
| 3 |
Fleenor, Elizabeth G (12)
|
VASFY |
36.91 |
36.43 |
-0.48 |
| 4 |
Caveness, Caroline J (12)
|
SEKP |
36.66 |
37.16 |
0.50 |
| 5 |
Moehrke, Taylor M (12)
|
NCEAC |
38.74 |
38.36 |
-0.38 |
| 6 |
McLaughlin, Delaney L (12)
|
VACCA |
41.97 |
38.44 |
-3.53 |
| 7 |
Holloway, Blythe M (12)
|
NCRSA |
41.89 |
39.50 |
-2.39 |
| 8 |
Bolling, Evelyn S (12)
|
VAGATR |
40.86 |
39.59 |
-1.27 |
| 9 |
Nunn, Malia A (12)
|
NCEAC |
49.69 |
41.62 |
-8.07 |
| 10 |
Ayers, Ansley E (12)
|
VACCA |
43.75 |
41.95 |
-1.80 |
| 11 |
Simkins, Elizabeth J (12)
|
VACCA |
|
43.55 |
- |
| 12 |
McMahon, Natalie E (12)
|
VASFY |
44.18 |
44.10 |
-0.08 |
| 13 |
MacKinnon, Sarah M (12)
|
NCRSA |
47.14 |
46.40 |
-0.74 |
| 14 |
Spradling, Leila C (12)
|
SEBSC |
51.13 |
47.06 |
-4.07 |
|
Kim, Catherine E (12)
|
NCEAC |
39.53 |
NS |
- |
|
Pienkowski, Nataleigh L (12)
|
VAHOKI |
45.20 |
NS |
- |
|
Faris, Lilliana S (12)
|
SEBSC |
|
NS |
- |
|
Ahart, Ryann A (12)
|
NCRSA |
51.79 |
NS |
- |