| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bradley, Isabelle B (12)
|
VACYAC |
35.71 |
34.63 |
-1.08 |
| 2 |
Smith, Hope A (12)
|
VACCA |
36.89 |
36.84 |
-0.05 |
| 3 |
Wall, Tatum E (12)
|
PVBWST |
39.12 |
37.58 |
-1.54 |
| 4 |
Smith, Morgan V (12)
|
VASFY |
37.21 |
37.60 |
0.39 |
| 5 |
Niculescu, Gabriela E (12)
|
VASMAC |
41.59 |
39.88 |
-1.71 |
| 6 |
Thompson, Caraline E (12)
|
VAHOKI |
40.53 |
40.51 |
-0.02 |
| 7 |
Berry, Jessica l (12)
|
VACYAC |
42.49 |
42.26 |
-0.23 |
| 8 |
Wright, Kara J (12)
|
VAHOKI |
45.23 |
44.31 |
-0.92 |
| 9 |
Pollard, Emily D (12)
|
VALASO |
45.87 |
45.76 |
-0.11 |
| 10 |
Ganter, Jamie C (12)
|
VAHOKI |
53.65 |
46.73 |
-6.92 |
| 11 |
Timm, Meredith R (12)
|
PVYORK |
47.55 |
48.27 |
0.72 |
| 12 |
Rice, Jolie M (12)
|
VAHOKI |
|
48.98 |
- |
|
Hudgins, Rebecca M (12)
|
VALASO |
47.14 |
NS |
- |
|
Csaszi, Lena E (12)
|
PVYORK |
|
NS |
- |