| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sipula, Estella A (11)
|
AMWHAT |
35.84 |
34.47 |
-1.37 |
| 2 |
Melocchi, Grace R (12)
|
AMSASC |
37.34 |
36.06 |
-1.28 |
| 3 |
Czajkowski, Anna E (12)
|
AMNAC |
39.46 |
39.78 |
0.32 |
| 4 |
Piraino, Maria A (12)
|
AMFAST |
39.17 |
39.80 |
0.63 |
| 5 |
Race, Delaney M (11)
|
AMMMSA |
39.92 |
39.83 |
-0.09 |
| 6 |
Laurenti, Gianna M (11)
|
AMMMSA |
40.13 |
41.12 |
0.99 |
| 7 |
Smith, Sydney (11)
|
AMGPAC |
43.09 |
41.88 |
-1.21 |
| 8 |
Greene, Emma M (12)
|
AMBCAT |
39.79 |
41.97 |
2.18 |
| 9 |
Miller, Laurana E (12)
|
AMMMSA |
42.66 |
42.36 |
-0.30 |
| 10 |
Van Norman, Ella C (11)
|
AMGLAC |
48.87 |
43.96 |
-4.91 |
| 11 |
Adzogba, Aiyanna A (12)
|
AMWHAT |
45.42 |
45.41 |
-0.01 |
| 12 |
Boone, Elizabeth A (12)
|
AMWHAT |
47.41 |
46.40 |
-1.01 |
| 13 |
Jackson, Julia R (11)
|
AMFAST |
52.45 |
47.65 |
-4.80 |
|
Bringhurst, Brenna M (11)
|
AMWHAT |
55.69 |
DQ |
- |
|
McPherson, Mackenzie S (11)
|
AMWHAT |
1:05.61 |
DQ |
- |
|
Ebbitt, Addison M (11)
|
AMWHAT |
57.69 |
NS |
- |