| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bell, Lauren C (17)
|
AMGLAC |
59.23 |
1:00.22 |
0.99 |
| 2 |
Holm, Aidah M (15)
|
AMFAST |
1:00.27 |
1:02.86 |
2.59 |
| 3 |
Smitley, Mikayla (17)
|
AMMPAC |
1:01.84 |
1:05.94 |
4.10 |
| 4 |
Peretti, Cameron S (14)
|
AMWHAT |
1:03.67 |
1:07.38 |
3.71 |
| 5 |
Tomasic, Anna M (13)
|
AMFAST |
1:11.99 |
1:07.53 |
-4.46 |
| 6 |
Boswell, Addison E (16)
|
AMFAST |
1:06.29 |
1:10.25 |
3.96 |
| 7 |
Boyd, Sydney M (14)
|
AMEFSC |
1:07.98 |
1:10.42 |
2.44 |
| 8 |
Carasia, Hannah J (15)
|
AMGLAC |
1:07.82 |
1:10.86 |
3.04 |
| 9 |
Brumbaugh, Lillian L (14)
|
AMWAAC |
1:15.13 |
1:12.66 |
-2.47 |
| 10 |
Obara, Julia R (14)
|
AMEFSC |
1:11.45 |
1:12.84 |
1.39 |
| 11 |
Holloway, Carson G (13)
|
AMWAAC |
1:17.02 |
1:14.03 |
-2.99 |
| 12 |
Wolford, Kate E (18)
|
AMGLAC |
1:10.42 |
1:14.21 |
3.79 |
| 13 |
Homan, Destini M (16)
|
AMGLAC |
1:16.37 |
1:15.35 |
-1.02 |
| 14 |
Davis, Nora K (13)
|
AMEFSC |
1:17.70 |
1:16.97 |
-0.73 |
| 15 |
Smith, Sydney G (15)
|
AMWHAT |
1:17.84 |
1:17.74 |
-0.10 |
| 16 |
Zakrzewski, Brinley C (14)
|
AMWAAC |
1:13.73 |
1:17.80 |
4.07 |
| 17 |
Defazio, Emily E (16)
|
AMWAAC |
1:27.26 |
1:18.81 |
-8.45 |
| 18 |
Miller, Olivia J (13)
|
AMGPAC |
1:19.91 |
1:19.26 |
-0.65 |
| 19 |
Cass, Delaney A (14)
|
AMWHAT |
1:21.93 |
1:23.95 |
2.02 |
| 20 |
Alessio, Madison A (16)
|
AMEFSC |
1:17.65 |
1:24.47 |
6.82 |
| 21 |
Baker, Riley A (14)
|
AMNAC |
1:30.90 |
1:24.75 |
-6.15 |