| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Spencer, Sarah O (14)
|
OHPCY |
29.64 |
29.49 |
-0.15 |
| 2 |
Donovan, Celia P (14)
|
OHPCY |
31.12 |
30.46 |
-0.66 |
| 3 |
Burke, Kathleen M (13)
|
OHPCY |
33.11 |
31.81 |
-1.30 |
| 4 |
Castenson, Zoe A (14)
|
OHBASH |
31.82 |
32.25 |
0.43 |
| 5 |
Jones, Ella E (13)
|
OHPCY |
34.60 |
32.65 |
-1.95 |
| 6 |
Wilver, Kaitlyn M (13)
|
OHABLY |
35.42 |
32.89 |
-2.53 |
| 7 |
Mundhe, Kimaya * (13)
|
OHBASH |
34.47 |
34.05 |
-0.42 |
| 8 |
Roberts, Anna E (13)
|
OHABLY |
33.40 |
34.35 |
0.95 |
| 9 |
McCauley, Gabriella M (13)
|
OHPCY |
35.27 |
34.82 |
-0.45 |
| 10 |
Janybek, Amina J (14)
|
OHBASH |
34.98 |
34.92 |
-0.06 |
| 11 |
Williams, Madalyn E (14)
|
OHPCY |
33.79 |
34.98 |
1.19 |
| 12 |
Chalmers, Lirabella S (13)
|
OHBASH |
37.32 |
35.70 |
-1.62 |
| 13 |
McKee, Bridget T (13)
|
OHABLY |
34.71 |
35.77 |
1.06 |
| 14 |
Auger, Annabel M (13)
|
OHPCY |
36.19 |
36.02 |
-0.17 |
| 15 |
McFarland, Ella G (14)
|
OHBASH |
40.38 |
37.76 |
-2.62 |
| 16 |
Suggs, Audry P (14)
|
OHABLY |
36.66 |
38.00 |
1.34 |
| 17 |
Finnerty, Shaela L (13)
|
OHBASH |
48.19 |
50.00 |
1.81 |