| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Huffman-Wynn, Maeley A (10)
|
OHCFY |
45.36 |
42.15 |
-3.21 |
| 2 |
Uhlman, Alyssa A (10)
|
OHBASH |
46.51 |
43.46 |
-3.05 |
| 3 |
Chalmers, Lirabella S (10)
|
OHBASH |
49.55 |
47.12 |
-2.43 |
| 4 |
Cain, Gabrielle E (9)
|
OHCFY |
49.67 |
47.38 |
-2.29 |
| 5 |
Stephens, Gabriella E (9)
|
OHRCDB |
50.61 |
49.36 |
-1.25 |
| 6 |
Mundhe, Kimaya * (10)
|
OHBASH |
56.85 |
49.75 |
-7.10 |
| 7 |
Leifheit, Grace E (9)
|
OHSPY |
52.89 |
50.13 |
-2.76 |
| 8 |
Wentzel, Charlotte M (9)
|
OHKEY |
53.47 |
50.95 |
-2.52 |
| 9 |
Lucas, Charlotte C (9)
|
OHRCDB |
47.68 |
51.44 |
3.76 |
| 10 |
Bennett, Emily R (9)
|
OHSPY |
56.08 |
52.42 |
-3.66 |
| 11 |
Auger, Annabel M (10)
|
OHPCY |
52.60 |
52.63 |
0.03 |
| 12 |
Bidleman, Sarah G (10)
|
OHRCDB |
49.93 |
53.00 |
3.07 |
| 13 |
Smith, Madison E (10)
|
OHKEY |
51.30 |
54.00 |
2.70 |
| 14 |
Shaffer, Alyssa N (10)
|
OHSPY |
53.89 |
54.39 |
0.50 |
| 15 |
Johannesmeyer, Rachel M (9)
|
OHPCY |
57.92 |
55.96 |
-1.96 |
| 16 |
Smith, Amelie R (9)
|
OHRCDB |
57.81 |
56.75 |
-1.06 |
| 17 |
Alvarado, Sarah S (9)
|
OHRCDB |
|
56.97 |
- |
| 18 |
Wallbrown, Katherine G (9)
|
OHBASH |
1:04.05 |
57.91 |
-6.14 |
| 19 |
Swanson-Bishop, Lucille R (9)
|
OHCFY |
|
59.15 |
- |
| 20 |
Morris, Madelynn B (10)
|
OHRCDB |
1:06.39 |
59.56 |
-6.83 |
| 21 |
Richardson, Lyla J (9)
|
OHRCDB |
|
1:01.66 |
- |
| 22 |
Detweiler, Yvette A (10)
|
OHBASH |
|
1:03.08 |
- |
| 23 |
Murphy, Molly M (9)
|
OHUCY |
|
1:04.51 |
- |
| 24 |
Kissick, Kalea E (10)
|
OHBASH |
|
1:04.83 |
- |
| 25 |
Bunn, Alexandra J (10)
|
OHBASH |
|
1:05.13 |
- |
| 26 |
Jinto, Irene (10)
|
OHBASH |
1:09.31 |
1:05.95 |
-3.36 |
| 27 |
Boller, Kathryn S (9)
|
OHCFY |
|
1:08.23 |
- |
| 28 |
Smal, Susanna S (10)
|
OHRCDB |
|
1:11.52 |
- |
|
Stepp, Raine M (10)
|
OHCFY |
|
NS |
- |
|
Johnson, McKenna A (10)
|
OHABLY |
47.75 |
NS |
- |