| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Uhlman, Alyssa A (10)
|
OHBASH |
45.66 |
45.48 |
-0.18 |
| 2 |
Huffman-Wynn, Maeley A (10)
|
OHCFY |
|
52.22 |
- |
| 3 |
Stephens, Gabriella E (9)
|
OHRCDB |
51.87 |
52.45 |
0.58 |
| 4 |
Cain, Gabrielle E (9)
|
OHCFY |
57.07 |
52.72 |
-4.35 |
| 5 |
Chalmers, Lirabella S (10)
|
OHBASH |
56.52 |
52.98 |
-3.54 |
| 6 |
Bidleman, Sarah G (10)
|
OHRCDB |
54.92 |
53.99 |
-0.93 |
| 7 |
Mundhe, Kimaya * (10)
|
OHBASH |
55.79 |
54.81 |
-0.98 |
| 8 |
Wentzel, Charlotte M (9)
|
OHKEY |
1:02.48 |
55.98 |
-6.50 |
| 9 |
Shaffer, Alyssa N (10)
|
OHSPY |
52.89 |
56.14 |
3.25 |
| 10 |
Bennett, Emily R (9)
|
OHSPY |
1:04.46 |
57.06 |
-7.40 |
| 11 |
Smith, Madison E (10)
|
OHKEY |
52.83 |
57.32 |
4.49 |
| 12 |
Boller, Kathryn S (9)
|
OHCFY |
|
1:00.38 |
- |
| 13 |
Smith, Amelie R (9)
|
OHRCDB |
1:03.41 |
1:00.82 |
-2.59 |
| 14 |
Jinto, Irene (10)
|
OHBASH |
1:04.25 |
1:02.15 |
-2.10 |
| 15 |
Auger, Annabel M (10)
|
OHPCY |
57.47 |
1:03.10 |
5.63 |
| 16 |
Detweiler, Yvette A (10)
|
OHBASH |
1:06.69 |
1:03.88 |
-2.81 |
| 17 |
Leifheit, Grace E (9)
|
OHSPY |
1:07.89 |
1:05.27 |
-2.62 |
| 18 |
Lucas, Charlotte C (9)
|
OHRCDB |
1:04.41 |
1:06.27 |
1.86 |
| 19 |
Wallbrown, Katherine G (9)
|
OHBASH |
1:08.43 |
1:07.81 |
-0.62 |
| 20 |
Johannesmeyer, Rachel M (9)
|
OHPCY |
|
1:08.47 |
- |
| 21 |
Kissick, Kalea E (10)
|
OHBASH |
1:11.04 |
1:09.12 |
-1.92 |
| 22 |
Swanson-Bishop, Lucille R (9)
|
OHCFY |
|
1:12.17 |
- |
| 23 |
Morris, Madelynn B (10)
|
OHRCDB |
|
1:15.29 |
- |
|
Alvarado, Sarah S (9)
|
OHRCDB |
|
DQ |
- |
|
Smal, Susanna S (10)
|
OHRCDB |
|
DQ |
- |
|
Richardson, Lyla J (9)
|
OHRCDB |
|
DQ |
- |
|
Murphy, Molly M (9)
|
OHUCY |
|
DQ |
- |
|
Stepp, Raine M (10)
|
OHCFY |
|
NS |
- |